Tissue Clearing Kit (Hydrophilic)

Product description: 

The product can be used for clearing of common animal tissues. Tissue clearing is achieved through delipidation and refractive index matching by immersing the tissue sequentially in the hydrophilic chemicals contained in this product following the instruction.

Product features: 

1. The product can be used to clear both intact and sectioned tissues. The cleared tissues are suitable for imaging with common microscopes, such as light sheet microscope and confocal microscope, etc..

2. The product has a high clearing efficiency. For instance, it usually takes 5-7 days to clear an adult mouse brain and ~2 minutes to clear a ~200 μm thick mouse brain slice. 

3. The product can be used to clear tissues with and without endogenous fluorophores. Genetically encoded fluorophores can be preserved with high efficiency after clearing. Fluorescent staining can be applied before or after delipidation following the instruction.

4. The cleared tissue has a high mechanical strength and preserve the cellular and subcellular structures effectively.

5. The product uses low toxicity hydrophilic chemicals for tissue clearing.

A mouse CNS before and after tissue clearing with the kit:

3D Image of the cleared mouse CNS

       Comparison of tissue clearing efficiency on P7 rabbit brains:

Imaging Case：

Thy1-GFP mouse brain

Anti-TH labeled mouse heart

Anti-TH labeled mouse liver

Experimental operation video：

Experimental operation video：

相關彩頁：

組織透明化試劑盒（親水型）-宣傳彩頁.pdf

親水性組織透明化試劑盒-使用說明書_2024.pdf

組織切片樣本透明化試劑盒-使用說明書.pdf

系列貨物清單：

Imaging Case Video：

Thy1-GFP成年小鼠完整中樞神經系統

采用锘海親水型透明化試劑盒制樣

分辨率：2μm×2μm×5μm

小鼠肺-自發(fā)熒光-3.2X

采用锘海親水型組織透明化試劑盒

分辨率：4 μm X 4 μm X 10.0 μm，成像尺寸：12.6 mm X 14.0 mm X 5.7 mm，拍攝時長：30 mins，放大倍數：3.2X

不同熒光染色的小鼠全腦

采用锘海親水型透明化試劑盒、锘海增強型透明化試劑盒

SM22:大血管 Lctin:微血管 TH: 多巴胺能神經元 Syto&P1: 細胞核

分辨率：4μmX4μmx10μm

了解更多透明化試劑盒相關信息

請聯系021-37827858 或 13818273779（微信同號）